nf-core/marsseq: systematic preprocessing pipeline for MARS-seq experiments
Proks M, Alejandro Romero Herrera J, Sedzinski J, Brickman JM (2025)
Publication Type: Journal article
Publication year: 2025
Journal
Book Volume: 5
Article Number: vbaf089
Journal Issue: 1
Abstract
Motivation: Single sequencing technology (scRNA-seq) enables the study of gene regulation at a single cell level. Although many sc-RNA-seq protocols have been established, they have varied in technical complexity, sequencing depth and multimodal capabilities leading to shared limitations in data interpretation due to a lack of standardized preprocessing and consistent data reproducibility. While plate based techniques such as Massively Parallel RNA Single cell Sequencing (MARS-seq2.0) provide reference data on the cells that will be sequenced, the data format limits the possible analysis. Here, we focus on the standardization of MARS-seq analysis and its applicability to RNA velocity. Results: We have taken the original MARS-seq2.0 pipeline and revised it to enable implementation using the nf-core framework. By doing so, we have simplified pipeline execution, enabling a streamlined application with increased transparency and scalability. We have incorporated additional checkpoints to verify experimental metadata and improved the pipeline by implementing a custom workflow for RNA velocity estimation. The pipeline is part of the nf-core bioinformatics community and is freely available at https://github.com/nfcore/marsseq with data analysis at https://github.com/brickmanlab/proks-et-al-2023.
Involved external institutions
Denmark (DK)How to cite
APA:
Proks, M., Alejandro Romero Herrera, J., Sedzinski, J., & Brickman, J.M. (2025). nf-core/marsseq: systematic preprocessing pipeline for MARS-seq experiments. Bioinformatics Advances, 5(1). https://doi.org/10.1093/bioadv/vbaf089
MLA:
Proks, Martin, et al. "nf-core/marsseq: systematic preprocessing pipeline for MARS-seq experiments." Bioinformatics Advances 5.1 (2025).
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